third generation helios mass cytometer (fluidigm)
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third generation helios mass cytometer
Third Generation Helios Mass Cytometer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 3453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+third+generation+mass+cytometer/Helios/bio_rxiv__2025__09__02__673852-86-5-9
Average 93 stars, based on 3453 article reviews
Third Generation Helios Mass Cytometer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 3453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/helios+third+generation+mass+cytometer/Helios/bio_rxiv__2025__09__02__673852-86-5-9
Average 93 stars, based on 3453 article reviews
third generation helios mass cytometer - by Bioz Stars,
2026-09
93/100 stars
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Cytometry:Article Title: Cellular junction and mesenchymal factors delineate an endometriosis-specific response of endometrial stromal cells to the mesothelium. Article Snippet: Metal conjugation to antibodies was carried out by the Maxpar® X8 Antibody Labeling Kit per manufacturer’s protocol (Fluidigm Corporation, San Francisco, CA) to generate the 28-marker adhesion and mesenchymal antibody panel. .. CyTOF was performed using the Article Title: Cellular junction and mesenchymal factors delineate an endometriosis-specific response of endometrial stromal cells to the mesothelium Article Snippet: Metal conjugation to antibodies was carried out by the Maxpar ® X8 Antibody Labeling Kit per manufacturer’s protocol (Fluidigm Corporation, San Francisco, CA) to generate the 28-marker adhesion and mesenchymal antibody panel. .. CyTOF was performed using the Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination Article Snippet: Cells were then incubated with Maxpar Perm-S buffer (Fluidigm, 201066) and TruStain Fc-X (1:20, Biolegend, 422302). .. The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10 min, gently resuspended in cold methanol and incubated on ice for another 10 min. After washing, cells were stained with the pooled nuclear antibodies at room temperature for 30 min and washed by Maxpar Cell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125 mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination. Article Snippet: The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room Nature Communications | (2023) 14:6569 17 temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10min, gently resuspended in cold methanol and incubated on ice for another 10min. .. After washing, cells were stained with the pooled nuclear antibodies at roomtemperature for 30min andwashedbyMaxparCell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Incubation:Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination Article Snippet: Cells were then incubated with Maxpar Perm-S buffer (Fluidigm, 201066) and TruStain Fc-X (1:20, Biolegend, 422302). .. The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10 min, gently resuspended in cold methanol and incubated on ice for another 10 min. After washing, cells were stained with the pooled nuclear antibodies at room temperature for 30 min and washed by Maxpar Cell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125 mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination. Article Snippet: The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room Nature Communications | (2023) 14:6569 17 temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10min, gently resuspended in cold methanol and incubated on ice for another 10min. .. After washing, cells were stained with the pooled nuclear antibodies at roomtemperature for 30min andwashedbyMaxparCell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Staining:Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination Article Snippet: Cells were then incubated with Maxpar Perm-S buffer (Fluidigm, 201066) and TruStain Fc-X (1:20, Biolegend, 422302). .. The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10 min, gently resuspended in cold methanol and incubated on ice for another 10 min. After washing, cells were stained with the pooled nuclear antibodies at room temperature for 30 min and washed by Maxpar Cell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125 mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination. Article Snippet: The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room Nature Communications | (2023) 14:6569 17 temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10min, gently resuspended in cold methanol and incubated on ice for another 10min. .. After washing, cells were stained with the pooled nuclear antibodies at roomtemperature for 30min andwashedbyMaxparCell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Software:Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination Article Snippet: Cells were then incubated with Maxpar Perm-S buffer (Fluidigm, 201066) and TruStain Fc-X (1:20, Biolegend, 422302). .. The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10 min, gently resuspended in cold methanol and incubated on ice for another 10 min. After washing, cells were stained with the pooled nuclear antibodies at room temperature for 30 min and washed by Maxpar Cell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125 mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using Article Title: Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination. Article Snippet: The cell surface and cytosol antibodies were pooled and added to the cells for incubation at room Nature Communications | (2023) 14:6569 17 temperature for 1 h. After washing with Maxpar Cell Staining Buffer (Fluidigm, 201068), cell pellets were chilled on ice for 10min, gently resuspended in cold methanol and incubated on ice for another 10min. .. After washing, cells were stained with the pooled nuclear antibodies at roomtemperature for 30min andwashedbyMaxparCell Staining Buffer (Fluidigm, 201068), then incubated with Maxpar Fix and Perm Buffer (Fluidigm, 201067) containing 0.125mM Cell-ID Intercalator-Ir 191/193 (Fluidigm, 201192A) at 4 °C for 16–18 h. Proteomic signals were measured using |